Part:BBa_K1036001

the ndh gene coding for respiratory NADH dehydrogenase II

Description

ndh is the coding gene for NADH dehydrogenase II (NCBI), a non-proton-translating single subunit membrane bond enzyme important in maintaining a redox-balanced dinucleotide pool. NADH dehydrogenase II is the preponderant respiratory source of H₂O₂ as well as O₂^-. In fact, substantial H₂O₂ was formed when quinoneless vesicles containing only NADH dehydrogenase II were incubated with NADH.
Earlier studies estimated that the formation rate of intracellular O₂^- was about 10^-5 M S^-1 by extrapolation of O₂^- yield from this enzyme. And NADH dehydrogenase II formed about 4 O₂^- per 10 molecules of H₂O₂ both during respiration and in the quinoneless membranes.
In our oscillatoral circuit, ndh just like a "conductor" because this gene could express NDH-2 which is a membrane-bound respiratory enzyme that produces low levels of H₂O₂ and superoxide. H₂O₂ will trigger synchronized oscillation between colonies.

Experimental Data

The ndh gene was amplifed from the genome DNA of E. coli strain BL21(DE3) via PCR. Then RBS(BBa_B0034), LVA-tag and terminator(BBa_B0015) were fused with ndh via fusion PCR becoming RBS-ndh-LVA-TT marked as ndh. The DH5α colony carried ndh gene was sequenced after TA-cloning.The Fig.1 showed the result of ndh gene fused with LVA-tag.

Sequence and Features

Barcodes are discontinued, but one was appended to the sequence of this part. Composite parts using this part will include the barcode. More ...

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 1360
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]